The Pentagon estimates it will cost about $30,000 per hour to operate the F-35, in large part due to the cost of fuel.
Although, given all the unreliable systems, if most of that is fuel, I expect the cost per flying hour to be much more. But let us explore their claim.
65 aircraft: 300 flight hours per year; $30,000 per flight hour; 30 years.
That is a lot more than what the DND stated for lifetime costs.